Bergquist N R, Nilsson P
Ann N Y Acad Sci. 1975 Jun 30;254:157-62. doi: 10.1111/j.1749-6632.1975.tb29165.x.
A pulsed laser microfluorometric system is a practical proposition. The narrow line of excitation makes filter quality less important. Because bleaching does not occur, reliable measurements and reexcitation of preparations for control purposes are feasible.
脉冲激光显微荧光测量系统是切实可行的。激发光的窄谱线使得滤光片质量不那么重要。由于不会发生漂白现象,因此进行可靠测量以及为控制目的对标本进行再激发都是可行的。