Goh D W, Momose Y, Middlesworth W, Hutson J M
Department of General Surgery, Royal Children's Hospital, Melbourne, Australia.
J Urol. 1993 Aug;150(2 Pt 2):574-6. doi: 10.1016/s0022-5347(17)35554-4.
The relationship among calcitonin gene-related peptide (CGRP), a neurotransmitter in the genitofemoral nerve, androgens and gubernacular development was studied using rats treated prenatally with the antiandrogen flutamide and the mutant cryptorchid TS rat. We compared these 2 groups with the testicular feminization mouse with androgen insensitivity. Gubernacula from male TS rats and flutamide-treated rats were maintained in organ culture and examined for contractile response to CGRP. Controls were gubernacula from normal rats and vehicle-treated rats, respectively. TS rat gubernacula have an inhibited contractile response to CGRP, whereas flutamide-treated rat gubernacula have an exaggerated response. A similar exaggerated response to CGRP has previously been demonstrated in testicular feminization mouse gubernacula. These results revealed abnormalities in gubernacular contractile response to CGRP in these cryptorchid animal models, implying that CGRP and gubernacular contractility may have key roles in mediating normal inguinoscrotal testicular descent.
利用产前接受抗雄激素氟他胺治疗的大鼠和隐睾突变TS大鼠，研究了生殖股神经中的神经递质降钙素基因相关肽（CGRP）、雄激素与睾丸引带发育之间的关系。我们将这两组与雄激素不敏感的睾丸雌性化小鼠进行了比较。将雄性TS大鼠和氟他胺处理大鼠的睾丸引带进行器官培养，并检测其对CGRP的收缩反应。对照组分别为正常大鼠和溶剂处理大鼠的睾丸引带。TS大鼠的睾丸引带对CGRP的收缩反应受到抑制，而氟他胺处理大鼠的睾丸引带反应过度。先前在睾丸雌性化小鼠的睾丸引带中也证明了对CGRP有类似的过度反应。这些结果揭示了这些隐睾动物模型中睾丸引带对CGRP的收缩反应异常，这意味着CGRP和睾丸引带的收缩性可能在介导正常的腹股沟阴囊睾丸下降中起关键作用。
