Zhang N, Ottersen O P
Anatomical Institute, University of Oslo, Norway.
Can J Neurol Sci. 1993 May;20 Suppl 3:S36-42.
Quantitative immunogold cytochemistry at the electron microscopic level was used to assess the endogenous contents of glutamate, aspartate, homocysteic acid, and glutamine (a precursor of glutamate) in the cerebellar climbing fiber terminals. Of the three excitatory amino acids, only glutamate appeared to be enriched in these terminals. The climbing fiber terminals also displayed immunoreactivity for glutamine. The level of aspartate immunoreactivity was far higher in the nerve cell bodies in the inferior olive than in their terminals in the cerebellar cortex. Homocysteic acid immunolabelling was concentrated in glial cells including the Golgi epithelial cells in the Purkinje cell layer. Our immunocytochemical data indicate that glutamate is a more likely climbing fiber transmitter than aspartate and homocysteic acid.
在电子显微镜水平上，采用定量免疫金细胞化学方法评估小脑攀缘纤维终末中谷氨酸、天冬氨酸、高半胱氨酸和谷氨酰胺（谷氨酸的前体）的内源性含量。在这三种兴奋性氨基酸中，只有谷氨酸似乎在这些终末中含量丰富。攀缘纤维终末对谷氨酰胺也表现出免疫反应性。天冬氨酸免疫反应性水平在下橄榄核的神经细胞体中比在小脑皮质的终末中要高得多。高半胱氨酸免疫标记集中在包括浦肯野细胞层中的高尔基上皮细胞在内的神经胶质细胞中。我们的免疫细胞化学数据表明，与天冬氨酸和高半胱氨酸相比，谷氨酸更有可能是攀缘纤维递质。
