Merten M D, Tournier J M, Meckler Y, Figarella C
INSERM U 14, Vandoeuvre lès Nancy, France.
Am J Respir Cell Mol Biol. 1993 Aug;9(2):172-8. doi: 10.1165/ajrcmb/9.2.172.
Submucosal gland cells isolated from human tracheas by enzymatic digestion and cultured in the absence or presence of epinephrine (E) were used to investigate the possible action of this catecholamine on the physiology of the gland secretory cells issued from the human trachea. A 3 x 10(-6) M concentration of E shortens the doubling time of growth and increases the cells' confluency rate. On the other hand, E appears to induce cell polarity in terms of differential secretion apically versus basolaterally. Furthermore, when human tracheal gland cells are cultured in the presence of E, a maximal cell stimulability by different agonists occurs from 8 days after confluency and then remains identical for 10 days, allowing us to compare the action of different adrenergic and cholinergic agonists on the proteinase bronchial inhibitor and the radiolabeled glycoconjugate secretion. As previously described, secretions of bronchial inhibitor and high molecular weight glycoconjugates were stimulated both by alpha- and beta-adrenergic and by cholinergic agonists but at a much higher rate when cells were cultured in the presence of E. These results indicate that E improves cultured human tracheal glandular cell growth and differentiation in that it increases their polarity and their ability to respond to adrenergic and cholinergic agonists.
通过酶消化从人气管分离并在无肾上腺素（E）或有肾上腺素存在的情况下培养的粘膜下腺细胞，被用于研究这种儿茶酚胺对源自人气管的腺分泌细胞生理学的可能作用。3×10⁻⁶ M浓度的E缩短了生长的倍增时间并提高了细胞的汇合率。另一方面，E似乎在顶端与基底外侧差异分泌方面诱导细胞极性。此外，当人气管腺细胞在E存在下培养时，不同激动剂对细胞的最大刺激作用在汇合后8天出现，然后在10天内保持相同，这使我们能够比较不同肾上腺素能和胆碱能激动剂对蛋白酶支气管抑制剂和放射性标记糖缀合物分泌的作用。如先前所述，支气管抑制剂和高分子量糖缀合物的分泌受到α-和β-肾上腺素能以及胆碱能激动剂的刺激，但当细胞在E存在下培养时，刺激速率要高得多。这些结果表明，E改善了培养的人气管腺细胞的生长和分化，因为它增加了它们的极性以及它们对肾上腺素能和胆碱能激动剂的反应能力。
