文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Department of Animal Sciences, Oregon State University, Corvallis 97331-6702.

Comp Biochem Physiol Comp Physiol. 1993 Jul;105(3):485-92. doi: 10.1016/0300-9629(93)90424-3.

Serum-containing media without additional hormones failed to support the differentiation of porcine SV cells while hormone-supplemented serum-free medium (ITTC) stimulated differentiation as indicated by higher GPDH specific activity. 2. All sera tested stimulated proliferation and inhibited porcine SV cell differentiation to some degree; fetal calf and rat sera were more inhibitory than calf and pig sera. 3. Morphologically, in serum-free medium porcine SV cells developed into individual adipocytes while in all serum-containing media they formed fat cell clusters. 4. Insulin and hydrocortisone in serum-free medium were important factors in SV cell differentiation while transferrin, T3, and fibroblast growth factor (FGF) were less important.

不含额外激素的含血清培养基无法支持猪SV细胞的分化，而添加激素的无血清培养基（ITTC）可刺激分化，较高的甘油磷酸脱氢酶（GPDH）比活性表明了这一点。2. 所有测试的血清均在一定程度上刺激增殖并抑制猪SV细胞分化；胎牛血清和大鼠血清的抑制作用比小牛血清和猪血清更强。3. 形态学上，在无血清培养基中猪SV细胞发育成单个脂肪细胞，而在所有含血清培养基中它们形成脂肪细胞簇。4. 无血清培养基中的胰岛素和氢化可的松是SV细胞分化的重要因素，而转铁蛋白、T3和成纤维细胞生长因子（FGF）的作用较小。

Department of Animal Sciences, Oregon State University, Corvallis 97331-6702.

Comp Biochem Physiol Comp Physiol. 1993 Jul;105(3):485-92. doi: 10.1016/0300-9629(93)90424-3.

Serum-containing media without additional hormones failed to support the differentiation of porcine SV cells while hormone-supplemented serum-free medium (ITTC) stimulated differentiation as indicated by higher GPDH specific activity. 2. All sera tested stimulated proliferation and inhibited porcine SV cell differentiation to some degree; fetal calf and rat sera were more inhibitory than calf and pig sera. 3. Morphologically, in serum-free medium porcine SV cells developed into individual adipocytes while in all serum-containing media they formed fat cell clusters. 4. Insulin and hydrocortisone in serum-free medium were important factors in SV cell differentiation while transferrin, T3, and fibroblast growth factor (FGF) were less important.

不含额外激素的含血清培养基无法支持猪SV细胞的分化，而添加激素的无血清培养基（ITTC）可刺激分化，较高的甘油磷酸脱氢酶（GPDH）比活性表明了这一点。2. 所有测试的血清均在一定程度上刺激增殖并抑制猪SV细胞分化；胎牛血清和大鼠血清的抑制作用比小牛血清和猪血清更强。3. 形态学上，在无血清培养基中猪SV细胞发育成单个脂肪细胞，而在所有含血清培养基中它们形成脂肪细胞簇。4. 无血清培养基中的胰岛素和氢化可的松是SV细胞分化的重要因素，而转铁蛋白、T3和成纤维细胞生长因子（FGF）的作用较小。