Cinque P, Brytting M, Vago L, Castagna A, Parravicini C, Zanchetta N, D'Arminio Monforte A, Wahren B, Lazzarin A, Linde A
Department of Virology, Karolinska Institute, Stockholm, Sweden.
Lancet. 1993 Aug 14;342(8868):398-401. doi: 10.1016/0140-6736(93)92814-a.
Epstein-Barr virus (EBV) is constantly associated with AIDS-related primary lymphomas of the central nervous system (CNS). To assess whether EBV DNA in cerebrospinal fluid (CSF) could be used as a tumour marker, CSF samples that had been taken within 180 days before death from 85 patients with HIV infection and neurological disorders at necropsy were examined retrospectively by nested polymerase chain reaction (PCR) for EBV. Histologically evident primary CNS lymphomas were found in 17 patients, and EBV was shown in tissue by in-situ hybridisation in 16 of the 16 cases examined. All 17 patients with primary CNS lymphoma had EBV DNA in CSF. EBV DNA was found in CSF from 1 of 68 HIV-infected patients without histologically detectable lymphoma at necropsy. PCR for EBV DNA in CSF was 100% sensitive and 98.5% specific for AIDS-associated primary CNS lymphoma, and may be useful as a diagnostic tumour marker.
爱泼斯坦-巴尔病毒（EBV）一直与艾滋病相关的中枢神经系统（CNS）原发性淋巴瘤有关。为了评估脑脊液（CSF）中的EBV DNA是否可作为肿瘤标志物，对85例在尸检前180天内死亡的HIV感染和神经系统疾病患者的脑脊液样本进行回顾性巢式聚合酶链反应（PCR）检测EBV。尸检时发现17例组织学上明显的原发性中枢神经系统淋巴瘤，在16例检测病例中，16例通过原位杂交在组织中显示有EBV。所有17例原发性中枢神经系统淋巴瘤患者的脑脊液中均有EBV DNA。在尸检时无组织学可检测到淋巴瘤的68例HIV感染患者中，有1例脑脊液中发现EBV DNA。脑脊液中EBV DNA的PCR检测对艾滋病相关原发性中枢神经系统淋巴瘤的敏感性为100%，特异性为98.5%，可能作为一种诊断性肿瘤标志物。
