肾上腺素能受体阻断诱导的内源性钠钾ATP酶激活因子

Endogenous factor activating Na,K-ATPase induced by blockade of adrenoceptors.

作者信息

Manukhin B N, Erokhov P A, Volina E V

机构信息

Institute of Developmental Biology, Russian Academy of Sciences, Moscow.

出版信息

Biochem Pharmacol. 1993 Jul 6;46(1):125-30. doi: 10.1016/0006-2952(93)90356-2.

DOI:10.1016/0006-2952(93)90356-2

PMID:8102232

Abstract

It was established that the regulatory factor released from the effector cells under the blockade of postsynaptic alpha- and beta-adrenoceptors by phentolamine and propranolol (0.5-1.0 microM) and activating noradrenergic uptake and synthesis increased the activity of Na,K-ATPase of isolated rat organs and partially purified Na,K-ATPase isolated from the rat brain. The regulatory factor is a protein with molecular mass 25-100 kDa, adsorbed on phenylsepharose CL-4B and eluated with 30 mM KCl. It consists of two active components with pI 5.1 and 5.9 which in admixture (1:1) activate Na,K-ATPase by 60%.

摘要

已确定，在酚妥拉明和普萘洛尔（0.5 - 1.0微摩尔）对突触后α和β肾上腺素能受体的阻断作用下，效应细胞释放的调节因子可激活去甲肾上腺素能摄取和合成，增加离体大鼠器官的钠钾ATP酶活性以及从大鼠脑中部分纯化的钠钾ATP酶的活性。该调节因子是一种分子量为25 - 100 kDa的蛋白质，吸附于苯基琼脂糖CL - 4B上，并用30 mM氯化钾洗脱。它由两种活性成分组成，其等电点分别为5.1和5.9，两者以1:1混合时可使钠钾ATP酶的活性提高60%。