Naess P A, Christensen G, Kiil F
University of Oslo, Institute for Experimental Medical Research, Ullevål Hospital, Norway.
Acta Physiol Scand. 1993 Jun;148(2):131-6. doi: 10.1111/j.1748-1716.1993.tb09542.x.
To investigate the effect of endothelin on renin release, experiments were performed in barbiturate-anaesthetized dogs with denervated kidneys. Intrarenal infusion of endothelin (1 ng min-1 kg-1 body wt) reduced renal blood flow (RBF) from 145 +/- 10 ml min-1 to 98 +/- 9 ml min-1 without altering renin release (1 +/- 1 microgram angiotensin I (AI) min-1). Renin release was then increased either by renal arterial constriction or ureteral occlusion. When renal arterial pressure was reduced to 50 mmHg, renin release averaged 79 +/- 20 micrograms AI min-1 in six dogs and fell significantly to 24 +/- 6 micrograms AI min-1 during endothelin infusion. During ureteral occlusion the inhibitory effect of endothelin on renin release either during inhibition of beta-adrenergic activity with propranolol or after inhibiting prostaglandin synthesis by indomethacin during intrarenal infusion of isoproterenol was examined. After propranolol administration ureteral occlusion increased renin release from 5 +/- 2 micrograms AI min-1 to 38 +/- 12 micrograms AI min-1 in six dogs. Subsequent intrarenal endothelin infusion (1 ng min-1 kg-1 body wt) during maintained ureteral occlusion reduced renin release to 10 +/- 3 micrograms AI min-1. In six other dogs prostaglandin synthesis was inhibited by indomethacin. Subsequent infusion of isoproterenol (0.2 microgram min-1 kg-1 body wt) to stimulate beta-adrenoceptor activity increased renin release from 13 +/- 4 micrograms AI min-1 to 68 +/- 8 micrograms AI min-1 during ureteral occlusion.(ABSTRACT TRUNCATED AT 250 WORDS)
为研究内皮素对肾素释放的影响，在巴比妥麻醉且肾去神经的犬身上进行了实验。肾内输注内皮素（1纳克·分钟⁻¹·千克⁻¹体重）使肾血流量（RBF）从145±10毫升·分钟⁻¹降至98±9毫升·分钟⁻¹，而肾素释放未改变（1±1微克血管紧张素I（AI）·分钟⁻¹）。随后通过肾动脉收缩或输尿管阻塞增加肾素释放。当肾动脉压降至50毫米汞柱时，6只犬的肾素释放平均为79±20微克AI·分钟⁻¹，在内皮素输注期间显著降至24±6微克AI·分钟⁻¹。在输尿管阻塞期间，研究了内皮素在通过普萘洛尔抑制β-肾上腺素能活性期间或在肾内输注异丙肾上腺素期间通过吲哚美辛抑制前列腺素合成后对肾素释放的抑制作用。给予普萘洛尔后，输尿管阻塞使6只犬的肾素释放从5±2微克AI·分钟⁻¹增加至38±12微克AI·分钟⁻¹。随后在持续输尿管阻塞期间肾内输注内皮素（1纳克·分钟⁻¹·千克⁻¹体重）使肾素释放降至10±3微克AI·分钟⁻¹。在另外6只犬中，吲哚美辛抑制了前列腺素合成。随后输注异丙肾上腺素（0.2微克·分钟⁻¹·千克⁻¹体重）以刺激β-肾上腺素能活性，在输尿管阻塞期间使肾素释放从13±4微克AI·分钟⁻¹增加至68±8微克AI·分钟⁻¹。（摘要截短于250字）
