Hussell T, Isaacson P G, Crabtree J E, Spencer J
Department of Histopathology, UCL Medical School, London, UK.
Lancet. 1993 Sep 4;342(8871):571-4. doi: 10.1016/0140-6736(93)91408-e.
An association has been shown between colonisation of gastric mucosa by Helicobacter pylori, acquisition of mucosa-associated lymphoid tissue (MALT), and occurrence of primary B-cell gastric MALT lymphoma. We investigated the immunological response of cells from 3 low-grade primary B-cell gastric MALT lymphomas to H pylori type NCTC 11637 and 12 isolates of H pylori from patients without lymphomas. After co-culture of tumour cells with bacteria, cells were examined for phenotypic evidence of activation and proliferation, and supernatant assayed to detect tumour-derived immunoglobulin and interleukin-2 (IL-2). Neoplastic B cells and non-neoplastic T cells proliferated, and IL-2-receptor expression by most cells in the cultures was increased with stimulating strains of H pylori. There were also increases in tumour immunoglobulin and IL-2 release when activation and proliferation were seen in response to stimulating bacteria. Removal of T cells from the tumour cell suspension reduced proliferation and IL-2-receptor expression. In comparison, no responses were seen in cells from high-grade gastric MALT lymphomas or low-grade B-cell MALT lymphomas of other sites. The response of low-grade B-cell gastric MALT lymphomas to stimulating strains of H pylori is dependent on H-pylori-specific T cells and their products, rather than the bacteria themselves.
幽门螺杆菌在胃黏膜的定植、黏膜相关淋巴组织（MALT）的获得与原发性B细胞胃MALT淋巴瘤的发生之间已显示出关联。我们研究了来自3例低度原发性B细胞胃MALT淋巴瘤的细胞对NCTC 11637型幽门螺杆菌以及12株来自无淋巴瘤患者的幽门螺杆菌的免疫反应。肿瘤细胞与细菌共培养后，检测细胞的激活和增殖的表型证据，并检测上清液以检测肿瘤衍生的免疫球蛋白和白细胞介素-2（IL-2）。肿瘤性B细胞和非肿瘤性T细胞增殖，并且培养物中大多数细胞的IL-2受体表达随着幽门螺杆菌刺激菌株而增加。当对刺激细菌有激活和增殖反应时，肿瘤免疫球蛋白和IL-2释放也增加。从肿瘤细胞悬液中去除T细胞可减少增殖和IL-2受体表达。相比之下，来自高度胃MALT淋巴瘤或其他部位的低度B细胞MALT淋巴瘤的细胞未见反应。低度B细胞胃MALT淋巴瘤对幽门螺杆菌刺激菌株的反应取决于幽门螺杆菌特异性T细胞及其产物，而非细菌本身。
