植物提取物中紫杉醇及紫杉醇类化合物的免疫分析

Immunoassay of taxol and taxol-like compounds in plant extracts.

作者信息

Leu J G, Jech K S, Wheeler N C, Chen B X, ERlanger B F

机构信息

Department of Pharmacology, Columbia University, College of Physicians and Surgeons, New York, N.Y. 10032.

出版信息

Life Sci. 1993;53(12):PL183-7. doi: 10.1016/0024-3205(93)90128-p.

DOI:10.1016/0024-3205(93)90128-p

PMID:8103185

Abstract

A mouse monoclonal anti-taxol antibody (69E4A8E) and a rabbit polyclonal anti-taxol antiserum were used to measure taxol levels in plant extracts in a double-blind experiment in conjunction with assays by HPLC. 69E4A8E was previously shown by ELISA to be specific for taxol with only a slight cross reaction with another bioactive compound, cephalomannine; the antiserum, on the other hand, was, by radioimmunoassay (RIA), essentially equally reactive with taxol and cephalomannine. Immunoassays of the plant extracts gave results in agreement with that found by HPLC, suggesting that the antibodies can be used in simple routine procedures for the quantification of taxol or taxol-like compounds in extracts of plants or other potential natural sources.

摘要

在一项双盲实验中，使用小鼠单克隆抗紫杉醇抗体（69E4A8E）和兔多克隆抗紫杉醇抗血清，结合高效液相色谱法（HPLC）测定植物提取物中的紫杉醇水平。酶联免疫吸附测定（ELISA）先前已表明69E4A8E对紫杉醇具有特异性，仅与另一种生物活性化合物三尖杉宁碱有轻微交叉反应；另一方面，通过放射免疫测定（RIA），该抗血清与紫杉醇和三尖杉宁碱的反应性基本相同。对植物提取物的免疫测定结果与HPLC的结果一致，这表明这些抗体可用于简单的常规程序，以定量植物提取物或其他潜在天然来源中的紫杉醇或类紫杉醇化合物。