红豆杉属植物树皮和针叶提取物中紫杉醇及相关紫杉烷的高效液相色谱法和胶束电动色谱法

High-performance liquid chromatography and micellar electrokinetic chromatography of taxol and related taxanes from bark and needle extracts of Taxus species.

作者信息

Chan K C, Alvarado A B, McGuire M T, Muschik G M, Issaq H J, Snader K M

机构信息

Program Resources, Inc./DynCorp, NCI-Frederick Cancer Research and Development Center, MD 21702.

出版信息

J Chromatogr B Biomed Appl. 1994 Jul 15;657(2):301-6. doi: 10.1016/s0378-4347(94)80006-5.

DOI:10.1016/s0378-4347(94)80006-5

PMID:7952094

Abstract

High-performance liquid chromatography (HPLC) and micellar electrokinetic chromatography (MEKC) were applied for the separation of taxol, cephalomannine, and baccatin III in crude extracts from the needle and bark of Taxus species. The chromatogram of the bark extract was cleaner than that of the needle allowing a more reliable detection of taxol and cephalomannine in the bark extract. However, HPLC quantitation of taxol in the needle extract would be difficult due to coeluting taxinines. Nevertheless, this was not a problem in the MEKC experiment. In comparison to HPLC, MEKC offered baseline resolution of taxol from taxinines in the needle extract, less solvent waste, a smaller sample requirement, and the simultaneous detection of taxol, cephalomannine and baccatin III in a relatively simpler electrophoretic run.

摘要

高效液相色谱法（HPLC）和胶束电动毛细管色谱法（MEKC）用于分离红豆杉属植物针叶和树皮粗提物中的紫杉醇、三尖杉宁碱和巴卡亭III。树皮提取物的色谱图比针叶提取物的更纯净，从而能够更可靠地检测树皮提取物中的紫杉醇和三尖杉宁碱。然而，由于紫杉醇宁与紫杉醇共洗脱，针叶提取物中紫杉醇的HPLC定量分析将很困难。不过，在MEKC实验中这不是问题。与HPLC相比，MEKC能够实现针叶提取物中紫杉醇与紫杉醇宁的基线分离，溶剂浪费更少，样品需求量更小，并且能在相对简单的电泳运行中同时检测紫杉醇、三尖杉宁碱和巴卡亭III。