Unsworth B R, Kaulenas M S
Differentiation. 1975 Aug 11;3(1-3):21-7. doi: 10.1111/j.1432-0436.1975.tb00842.x.
Ribosomes isolated from unfertilised eggs of the sea urchin, Strongylocentrotus purpuratus, have a higher protein: RNA ratio than ribosomes extracted from blastula stage ribosomes. Approximately 64 additional protein equivalents are found per ribosome. Most of the proteins are of high molecular weight and are tightly bound, being resistant to high-salt and EDTA treatment. The majority of the proteins appear to be basic in nature and remain associated with the 40S subunit on dissociation of the ribosomes. The possible physiological significance of the additional proteins is discussed in terms of the activation of protein synthesis following fertilisation. Sea urchin ribosomes, isolated from various stages of development, showed differential protein-labelling patterns. The high molecular-weight proteins had preferentially higher specific activities and one ribosomal protein was particularly highly labelled, reaching a maximum at the gastrula stage of development. The functional role of this highly labelled protein during development is discussed.
从紫海胆未受精卵中分离出的核糖体，其蛋白质与RNA的比例高于从囊胚期提取的核糖体。每个核糖体大约还发现了64个额外的蛋白质当量。大多数蛋白质分子量高且紧密结合，耐高盐和EDTA处理。大多数蛋白质在性质上似乎是碱性的，在核糖体解离时仍与40S亚基结合。从受精后蛋白质合成激活的角度讨论了这些额外蛋白质可能的生理意义。从发育的各个阶段分离出的海胆核糖体显示出不同的蛋白质标记模式。高分子量蛋白质具有优先更高的比活性，并且一种核糖体蛋白标记特别高，在发育的原肠胚阶段达到最大值。讨论了这种高标记蛋白质在发育过程中的功能作用。
