黑腹果蝇衰老过程中线粒体DNA的丢失

Loss of mitochondrial DNA with aging in Drosophila melanogaster.

作者信息

Massie H R, Baird M B, McMahon M M

出版信息

Gerontologia. 1975;21(4):231-8.

Abstract

The buoyant densities of nuclear and mitochondrial DNA from Drosophila melanogaster lysates has been found to show no change with increasing age in both CsCl and Cs2SO4 equilibrium density gradients. Whole fly homogenates were used to demonstrate no change in nuclear DNA content during adult life. Mitochondrial DNA increased from 1.2 to 4.3% of the total DNA during the first week of adult life and then decreased during senescence to a minimum of 1.5% at 10 weeks of age which represented a 65% loss in mitochondrial DNA content with age. These data are interpreted to support the proposal that mitochondria destruction occurs during senescence.

摘要

在氯化铯（CsCl）和硫酸铯（Cs2SO4）平衡密度梯度中，已发现来自黑腹果蝇裂解物的核DNA和线粒体DNA的浮力密度不会随年龄增长而发生变化。使用全蝇匀浆来证明成年期核DNA含量没有变化。线粒体DNA在成年后的第一周从总DNA的1.2%增加到4.3%，然后在衰老过程中减少，在10周龄时降至最低1.5%，这表明线粒体DNA含量随年龄增长减少了65%。这些数据被解释为支持衰老过程中线粒体发生破坏这一观点。