ω-3脂肪酸与氯贝丁酯或邻苯二甲酸二（2-乙基己基）酯对雄性Wistar大鼠肝脏过氧化物酶体增殖的相互作用潜力。

Interactive potential of omega-3 fatty acids with clofibrate or DEHP on hepatic peroxisome proliferation in male Wistar rats.

作者信息

Wysynski A M, Baldwin L A, Leonard D A, Calabrese E J

机构信息

Environmental Health Sciences Program, School of Public Health, University of Massachusetts, Amherst 01003.

出版信息

Hum Exp Toxicol. 1993 Jul;12(4):337-40. doi: 10.1177/096032719301200413.

DOI:10.1177/096032719301200413

PMID:8104013

Abstract

The interactive potential of three known peroxisome proliferators, omega-3 fatty acids, clofibrate and di(2-ethylhexylphthalate (DEHP), was evaluated in male weanling Wistar rats for the effect on peroxisomal beta-oxidation. Omega-3 fatty acids were supplied by menhaden oil which was fed in six regimens: low fat (5% w/w), low fat and clofibrate (0.3% w/w) or DEHP (0.25% w/w), high fat (20% w/w), high fat and clofibrate or DEHP in the aforementioned concentrations. Induction of peroxisomal beta-oxidation was measured by changes in liver-to-body weight ratio, fatty acyl-CoA oxidase (FAO) activity, and peroxisomal bifunctional enzyme (PBE) quantity. Analysis of transformed data indicated a less than additive response in FAO activity with no deviation from additivity seen with liver-to-body ratios and PBE.

摘要

在雄性断乳Wistar大鼠中评估了三种已知的过氧化物酶体增殖剂（ω-3脂肪酸、氯贝丁酯和邻苯二甲酸二（2-乙基己基）酯（DEHP））对过氧化物酶体β-氧化作用的交互潜力。ω-3脂肪酸由Menhaden油提供，以六种方案喂食：低脂（5% w/w）、低脂加氯贝丁酯（0.3% w/w）或DEHP（0.25% w/w）、高脂（20% w/w）、高脂加上述浓度的氯贝丁酯或DEHP。通过肝体重比、脂肪酰基辅酶A氧化酶（FAO）活性和过氧化物酶体双功能酶（PBE）数量的变化来测量过氧化物酶体β-氧化的诱导情况。对转换后数据的分析表明，FAO活性的反应小于相加性，而肝体重比和PBE未出现偏离相加性的情况。