Nishi K, Yoshida M, Horinouchi S, Beppu T
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Arch Microbiol. 1993;160(2):162-5. doi: 10.1007/BF00288720.
The pmd1+, a multidrug resistance gene of the fission yeast Schizosaccharomyces pombe, encodes a protein similar to the budding yeast Saccharomyces cerevisiae STE6 gene product and mammalian P-glycoproteins. The STE6 protein is a membrane transporter of a-factor, a mating pheromone of a-type S. cerevisiae, which is structurally related to M-factor of the fission yeast. However, heterothallic or homothallic pmd1 null mutant cells of S. pombe, which were constructed by means of gene disruption, showed no significant decrease in the mating abilities. On the other hand, the multidrug resistance conferred by the pmd1+ was overcome by the treatment with verapamil, a typical inhibitor of mammalian P-glycoproteins. These results indicate that the pmd1+ gene product is functionally similar to mammalian P-glycoproteins, rather than to the budding yeast STE6.
pmd1+是裂殖酵母粟酒裂殖酵母的一种多药耐药基因，编码一种与出芽酵母酿酒酵母STE6基因产物和哺乳动物P-糖蛋白相似的蛋白质。STE6蛋白是a-因子的膜转运体，a-因子是a型酿酒酵母的一种交配信息素，其结构与裂殖酵母的M-因子相关。然而，通过基因敲除构建的粟酒裂殖酵母异宗配合或同宗配合的pmd1缺失突变体细胞，其交配能力没有显著下降。另一方面，pmd1+赋予的多药耐药性可被维拉帕米（一种典型的哺乳动物P-糖蛋白抑制剂）处理所克服。这些结果表明，pmd1+基因产物在功能上与哺乳动物P-糖蛋白相似，而不是与出芽酵母STE6相似。
