谷氨酰胺合成酶双循环级联反应中尿苷酰化和腺苷酰化状态的时间进程。
Time course of the uridylylation and adenylylation states in the glutamine synthetase bicyclic cascade.
作者信息
Varón-Castellanos R, Havsteen B H, García-Moreno M, Valero-Ruiz E, Molina-Alarcón M, García-Cánovas F
机构信息
Departamento de Química-Física, Escuela Universitaria Politécnica, Universidad de Castilla-La Mancha, Albacete, Spain.
出版信息
Biochem J. 1993 Sep 15;294 ( Pt 3)(Pt 3):813-9. doi: 10.1042/bj2940813.
A kinetic analysis of the glutamine synthetase bicyclic cascade is presented. It includes the dependence on time from the onset of the reaction of both the uridylylation of Shapiro's regulatory protein and the adenylylation of the glutamine synthetase. The transient phase equations obtained allow an estimation of the time elapsed until the states of uridylylation and adenylylation reach their steady-states, and therefore an evaluation of the effective sensitivity of the system. The contribution of the uridylylation cycle to the adenylylation cycle has been studied, and an equation relating the state of adenylylation at any time to the state of uridylylation at the same instant has been derived.
本文对谷氨酰胺合成酶双环级联反应进行了动力学分析。它包括从反应开始时起，夏皮罗调节蛋白的尿苷酰化和谷氨酰胺合成酶的腺苷酰化对时间的依赖性。所得到的瞬态相方程能够估计直到尿苷酰化和腺苷酰化状态达到其稳态所经过的时间，从而对系统的有效敏感性进行评估。研究了尿苷酰化循环对腺苷酰化循环的贡献，并推导了一个将任意时刻的腺苷酰化状态与同一时刻的尿苷酰化状态相关联的方程。
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