Karwinski W, Ulvik R, Farstad M, Svardal A, Berge R, Søreide O
Department of Surgery, Haukeland Hospital, University of Bergen, Norway.
Eur J Surg. 1993 Jun-Jul;159(6-7):355-9.
To find out whether oxygen free radical liberated by activation of xanthine oxidase change the tissue concentration of glutathione.
Controlled study.
42 male Wistar rats.
Laparotomy, induction of ischemia, and reperfusion. 27 rats were treated with allopurinol (to inhibit xanthine oxidase) and the remaining 15 acted as controls.
Concentrations of reduced glutathione, oxidized glutathione, and total glutathione in hepatocytes, blood, and bile.
Concentration of reduced and total glutathione in hepatocytes decreased significantly during reperfusion and oxidized glutathione was unchanged in all groups. Total glutathione in peripheral venous blood was reduced by half during the period of ischemia and increased gradually during reperfusion whereas the concentration of total glutathione in bile decreased appreciable during reperfusion. Production of bile improved significantly during reperfusion in the group treated with allopurinol compared with the control group.
Xanthine oxidase may not be the main source of production of oxygen free radicals as allopurinol did not affect the hepatic concentration of glutathione during reperfusion.
探究黄嘌呤氧化酶激活后释放的氧自由基是否会改变谷胱甘肽的组织浓度。
对照研究。
42只雄性Wistar大鼠。
剖腹手术、诱导缺血和再灌注。27只大鼠用别嘌呤醇治疗（以抑制黄嘌呤氧化酶），其余15只作为对照。
肝细胞、血液和胆汁中还原型谷胱甘肽、氧化型谷胱甘肽和总谷胱甘肽的浓度。
再灌注期间肝细胞中还原型和总谷胱甘肽浓度显著降低，所有组中氧化型谷胱甘肽无变化。外周静脉血中的总谷胱甘肽在缺血期间减少一半，在再灌注期间逐渐增加，而胆汁中总谷胱甘肽的浓度在再灌注期间明显降低。与对照组相比，用别嘌呤醇治疗的组在再灌注期间胆汁分泌显著改善。
黄嘌呤氧化酶可能不是氧自由基产生的主要来源，因为别嘌呤醇在再灌注期间不影响肝脏中谷胱甘肽的浓度。
