Pacciarini M L, Savio M L, Donini G, Tagliabue S
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
Rev Sci Tech. 1993 Jun;12(2):647-63. doi: 10.20506/rst.12.2.711.
The authors describe work in progress at the laboratory in Brescia, Italy, on the application of molecular methods to the diagnosis of leptospirosis. This work includes the following: a) Development of polymerase chain reaction (PCR) assays capable of amplifying specific deoxyribonucleic acid fragments from most Leptospira interrogans strains. b) Development of a microtitre-based assay for rapid detection of PCR-positive samples. c) Characterisation of Leptospira strains through restriction endonuclease analysis of PCR products and amplified fragment length polymorphism.
作者们描述了意大利布雷西亚实验室正在进行的关于分子方法在钩端螺旋体病诊断中的应用的工作。这项工作包括以下内容：a）开发能够从大多数问号钩端螺旋体菌株中扩增特定脱氧核糖核酸片段的聚合酶链反应（PCR）检测方法。b）开发一种基于微量滴定板的检测方法，用于快速检测PCR阳性样本。c）通过对PCR产物的限制性内切酶分析和扩增片段长度多态性对钩端螺旋体菌株进行特征分析。
