Pilgrim D
MRC Laboratory of Molecular Biology, Cambridge, U.K.
Genome. 1993 Aug;36(4):712-24. doi: 10.1139/g93-096.
A genetic approach was taken to identify new transposable element Tc1-dependent polymorphisms on the left end of linkage group III in the nematode Caenorhabditis elegans. The cloning of the genomic DNA surrounding the Tc1 allowed the selection of overlapping clones (from the collection being used to assemble the physical map of the C. elegans genome). A contig of approximately 600-800 kbp in the region has been identified, the genetic map of the region has been refined, and 10 new RFLPs as well as at least four previously characterized genetic loci have been positioned onto the physical map, to the resolution of a few cosmids. This analysis demonstrated the ability to combine physical and genetic mapping for the rapid analysis of large genomic regions (0.5-1 Mbp) in genetically amenable eukaryotes.
采用遗传学方法来鉴定线虫秀丽隐杆线虫连锁群III左端新的依赖转座元件Tc1的多态性。围绕Tc1的基因组DNA的克隆使得能够从用于构建秀丽隐杆线虫基因组物理图谱的文库中选择重叠克隆。已鉴定出该区域约600 - 800 kbp的重叠群，该区域的遗传图谱得到了完善，10个新的限制性片段长度多态性（RFLP）以及至少四个先前已鉴定的遗传位点已定位到物理图谱上，分辨率达到了几个黏粒的水平。该分析证明了在遗传上易于操作的真核生物中，能够将物理图谱和遗传图谱相结合，以快速分析大的基因组区域（0.5 - 1 Mbp）。
