Scales R W, Jacobs N F, Skaggs R
J Clin Microbiol. 1975 Oct;2(4):292-5. doi: 10.1128/jcm.2.4.292-295.1975.
Preparations of purified immunoglobulins, light chains, and unrelated proteins (used as controls) were covalently linked to agarose beads and used to study the specificity of fluorescein isothiocyanate conjugates. The data demonstrate how these beads can be used to detect immunological and non-immunological reactivity in conjugates. Commercial conjugates, conjugates prepared in this laboratory, and fluorescein-labeled normal immunoglobulins demonstrated high reactivity with control beads unless chromatographed on diethylaminoethyl-cellulose to select for the proper fluorescein-protein ratio. Undesirable immunological reactivity could be demonstrated in commercial conjugates and was shown to be due to anti-light-chain antibody.
将纯化的免疫球蛋白、轻链和无关蛋白质（用作对照）制剂共价连接到琼脂糖珠上，并用于研究异硫氰酸荧光素缀合物的特异性。数据表明这些珠子可如何用于检测缀合物中的免疫和非免疫反应性。市售缀合物、本实验室制备的缀合物以及荧光素标记的正常免疫球蛋白与对照珠子表现出高反应性，除非在二乙氨基乙基纤维素上进行色谱分离以选择合适的荧光素 - 蛋白质比例。在市售缀合物中可证明存在不良免疫反应性，并且已表明这是由于抗轻链抗体所致。
