Noble R C, Penny B B
J Lab Clin Med. 1975 Nov;86(5):713-21.
Group A streptococcal M protein is defined by its ability to stimulate type-specific precipitating and protective antibodies. Type 12 M protein was prepared by the acid-heat extraction of whole cells and ammonium sulfate precipitation. Acrylamide gel electrophoresis of the resulting M protein revealed multiple protein bands. The acrylamide gel was divided into three parts and protein from each part was tested for the ability to stimulate antibodies in rabbits. Only the proximal two portions of the gel produced protective antibody as measured by the long-chain and mouse-protective tests. Protein in all sections of the gel stimulated the production of precipitating and hemagglutinating antibodies. A low level of protective antibody as measured by the indirect bactericidal test was present only in the antisera to Part 1 of the gel. The results indicate that M protein stimulates antibodies with different functions.
A组链球菌M蛋白是由其刺激型特异性沉淀抗体和保护性抗体的能力所定义的。12型M蛋白是通过对全细胞进行酸热提取和硫酸铵沉淀制备的。所得M蛋白的丙烯酰胺凝胶电泳显示出多条蛋白带。将丙烯酰胺凝胶分成三部分，并检测每部分的蛋白刺激兔体内抗体产生的能力。通过长链和小鼠保护试验测定，只有凝胶近端的两部分产生了保护性抗体。凝胶所有部分的蛋白都刺激了沉淀抗体和血凝抗体的产生。通过间接杀菌试验测定，只有针对凝胶第1部分的抗血清中存在低水平的保护性抗体。结果表明M蛋白刺激产生具有不同功能的抗体。
