Reeves O R
J Embryol Exp Morphol. 1975 Aug;34(1):55-73.
The keratin-like proteins (KLPs) from the epidermis of adult frogs of the species Xenopus laevis have been isolated and biochemically characterized by means of polyacrylamide gel electrophoresis, amino acid analysis, tryptic peptide mapping, amino-terminal end-group analysis and isoelectric focusing. One particular protein fraction of rather unusual amino acid composition found only in epidermal tissue was isolated in quantity by preparative gel electrophoresis and monospecific antibodies prepared against it. Using this anti-KLP antibody preparation it was possible to show that at least one kine of keratin-like protein characteristic of the adult epidermis first appears within the larval epidermis during metamorphosis. This is the first reported biochemical characterization of a tissue-specific protien from adult amphibian skin.
非洲爪蟾成年蛙表皮中的类角蛋白（KLPs）已通过聚丙烯酰胺凝胶电泳、氨基酸分析、胰蛋白酶肽图谱分析、氨基末端基团分析和等电聚焦进行了分离和生化表征。通过制备性凝胶电泳大量分离出一种仅在表皮组织中发现的具有相当不寻常氨基酸组成的特定蛋白质组分，并制备了针对它的单特异性抗体。使用这种抗KLP抗体制剂，可以证明至少一种成年表皮特有的类角蛋白在变态过程中首次出现在幼虫表皮内。这是首次报道的成年两栖动物皮肤组织特异性蛋白质的生化表征。
