Nagasawa H, Kogure K, Ido T
Department of Neurology, Institute of Brain Diseases, Tohoku University School of Medicine, Sendai, Japan.
Tohoku J Exp Med. 1993 Jan;169(1):87-9. doi: 10.1620/tjem.169.87.
Neurotransmitter and receptor systems of the rat brain were simultaneously observed by in vivo double autoradiography using a novel imaging plate analyzer system which was developed by Fuji Photo Film Co., Ltd. The animals were subjected to intravenous administration of [18F]-6-fluoro-L-dopa ([18F]-DOPA) and [3H]YM-09151-2, a highly selective dopamine D2 antagonist. The radioactivities of [18F]-DOPA and [3H]YM-09151-2 were found to be highly concentrated in the striatum, demonstrating presynaptic and postsynaptic sites of the dopaminergic nigrostriatal tract, respectively. The [3H]YM-09151-2 was also found to be accumulated in the substantia nigra which is known to correspond with the distribution of dopamine D2 receptors. This method was shown to be useful for investigation of animal models with brain diseases.
利用富士胶片株式会社开发的新型成像板分析仪系统，通过体内双放射自显影技术同时观察大鼠脑内的神经递质和受体系统。给动物静脉注射[18F]-6-氟-L-多巴（[18F]-DOPA）和[3H]YM-09151-2（一种高选择性多巴胺D2拮抗剂）。发现[18F]-DOPA和[3H]YM-09151-2的放射性高度集中在纹状体，分别显示了多巴胺能黑质纹状体通路的突触前和突触后位点。还发现[3H]YM-09151-2在黑质中积累，已知黑质与多巴胺D2受体的分布相对应。该方法被证明可用于研究患有脑部疾病的动物模型。
