Yonemura Y, Fushida S, Tsugawa K, Ninomiya I, Fonseca L, Yamaguchi A, Miyazaki I, Urano T, Shiku H
Second Department of Surgery, Kanazawa University, School of Medicine, Japan.
Anal Cell Pathol. 1993 Sep;5(5):277-88.
We analyzed immunohistochemically the association between p53 tissue status and prognostic parameters of 357 gastric cancer patients, using endoscopically obtained biopsy materials, embedded in paraffin. Using PAb1801, an anti-p53 monoclonal antibody p53 immunoreactivity was detected in the nuclei of cancer cells in 113 cases (32%). The nuclear p53 immunoreaction was closely associated with positive lymph node metastasis, serosal invasion, and liver metastasis. For the estimation of proliferative activity, proliferating cell nuclear antigen (PCNA) labeling rates (LRs) of biopsy specimens were immunohistochemically measured. A significant positive correlation was found between PCNA LRs and p53 tissue status. In addition, a positive nuclear p53 immunoreaction was found to be significantly associated with shorter overall survival. Especially, in the group of patients with stages III and IV, the prognosis of patients with p53-positive tumours was significantly poorer than that of patients with p53-negative tumours. These results indicate that immunohistochemical staining for nuclear p53 in biopsied materials may be useful in deciding the therapeutic schedule of patients with gastric cancer, preoperatively.
我们使用经内镜获取的、石蜡包埋的活检材料，对357例胃癌患者的p53组织状态与预后参数之间的关联进行了免疫组织化学分析。使用抗p53单克隆抗体PAb1801，在113例（32%）患者的癌细胞核中检测到p53免疫反应性。细胞核p53免疫反应与阳性淋巴结转移、浆膜侵犯及肝转移密切相关。为评估增殖活性，采用免疫组织化学方法测定活检标本的增殖细胞核抗原（PCNA）标记率（LRs）。发现PCNA LRs与p53组织状态之间存在显著正相关。此外，细胞核p53免疫反应阳性与总生存期较短显著相关。特别是在III期和IV期患者组中，p53阳性肿瘤患者的预后明显差于p53阴性肿瘤患者。这些结果表明，术前对活检材料进行细胞核p53免疫组织化学染色可能有助于确定胃癌患者的治疗方案。
