Ono K, Abe J, Kagawa N, Ii K, Hizawa K
First Department of Pathology, University of Tokushima School of Medicine, Japan.
Zentralbl Pathol. 1993 Aug;139(3):231-7.
We investigated the onset and duration of DNA synthesis and differentiation of muscle precursor cells in the early stage in muscle regeneration after bupivacaine induced muscle necrosis using monoclonal anti-PCA and anti-desmin antibodies. The first positive PCNA reaction indicating the commencement of proliferating cycle of muscle precursor cells appeared at 24 hours after injury, and were most prominent at 60-72 hours after injury. Thereafter the reaction for PCNA disappeared as the maturation of regenerated myofibers progressed. Some nuclei of regenerated myotubes in the early stage were positive for PCNA contrary to the generally accepted concept that myonuclei after fusion were out of cell cycle. The first desmin expression was observed in mononuclear presumptive myoblasts at 48 hours after injury, some of which were also positive for PCNA in the double immunostained sections for desmin and PCNA. This result suggested that differentiation of presumptive myoblasts could already initiate during their proliferative phase.
我们使用单克隆抗增殖细胞核抗原（PCNA）抗体和抗结蛋白抗体，研究了布比卡因诱导肌肉坏死后早期肌肉再生过程中肌肉前体细胞DNA合成和分化的起始及持续时间。表明肌肉前体细胞增殖周期开始的首个阳性PCNA反应在损伤后24小时出现，并在损伤后60 - 72小时最为显著。此后，随着再生肌纤维成熟，PCNA反应消失。早期再生肌管的一些细胞核PCNA呈阳性，这与通常认为融合后的肌细胞核脱离细胞周期的概念相反。在损伤后48小时，在单核假定成肌细胞中观察到首个结蛋白表达，其中一些在结蛋白和PCNA的双重免疫染色切片中PCNA也呈阳性。这一结果表明假定成肌细胞的分化可能在其增殖阶段就已开始。
