Broqua P, Benyassi A, Grouselle D, Arancibia S
Laboratoire de Pharmacologie, CNRS URA 1482, CHU Necker-E.M., Paris, France.
Neuroreport. 1993 Sep 3;4(10):1200-2.
Acute administration of the serotonin (5-HT)1A receptor agonist ipsapirone has been previously shown to elicit a dose-dependent decrease of cold-elicited thyrotropin (TSH) secretion. Therefore, the aim of the present study was to evaluate, by means of a push-pull cannula implanted in the median eminence (ME), whether ipsapirone acts primarily through an inhibition of cold-elicited thyrotropin-releasing hormone (TRH) release. Ipsapirone administration (10 mg kg-1 i.p., 30 min before cold) prevented cold-elicited TRH release, thereby confirming the above hypothesis. In addition, this study further validates push-pull perfusion in the ME as a key tool for measuring TRH secretion in vivo.
先前的研究表明，急性给予5-羟色胺（5-HT）1A受体激动剂伊沙匹隆可引起冷刺激促甲状腺激素（TSH）分泌呈剂量依赖性降低。因此，本研究旨在通过植入正中隆起（ME）的推挽式套管评估伊沙匹隆是否主要通过抑制冷刺激促甲状腺激素释放激素（TRH）的释放发挥作用。给予伊沙匹隆（10 mg kg-1腹腔注射，冷刺激前30分钟）可防止冷刺激引起的TRH释放，从而证实了上述假设。此外，本研究进一步验证了ME中的推挽式灌注作为体内测量TRH分泌的关键工具的有效性。
