Léonce S, Burbridge M
Institut de Recherches Servier, Division de Cancérologie Expérimentale, Suresnes, France.
Biol Cell. 1993;78(1-2):63-8. doi: 10.1016/0248-4900(93)90115-u.
Flow cytometry has recently become a very important tool in the study of the mechanisms of multidrug resistance (MDR). This technique allows rapid access to information regarding two characteristics related to the MDR phenotype: i) the level of overexpression of the P-glycoprotein; and ii) its functional aspect in expulsion of the cytotoxic agents to which the cell is exposed. In pharmacology, flow cytometry also allows evaluation of the capacity of modulating agents to reverse this resistance, and contributes a deeper insight into the mechanism of action of new molecules.
流式细胞术最近已成为研究多药耐药（MDR）机制的一项非常重要的工具。这项技术能够快速获取与MDR表型相关的两个特征的信息：i）P-糖蛋白的过表达水平；ii）其在将细胞暴露于的细胞毒性药物排出方面的功能情况。在药理学中，流式细胞术还能够评估调节剂逆转这种耐药性的能力，并有助于更深入地了解新分子的作用机制。
