Itagaki T, Yoshida M, Abe S, Omichi H, Nishihira Y
Seed Co., Ohmiya Laboratory, Saitama, Japan.
J Chromatogr. 1993 Oct 22;620(1):149-52. doi: 10.1016/0378-4347(93)80063-a.
Human tear protein, which consists mainly of albumin, lysozyme, and lactoferrin, was assayed with high-performance liquid chromatography using a new ceramic hydroxyapatite column. Proteins were eluted at room temperature using a 20-min linear gradient from 95:5% A/B buffer to 0:100% A/B buffer (buffer A, distilled water; buffer B, 400 mM KH2PO4 containing 240 mM NaOH). The proteins eluted at 1.2 min for albumin, 8.5 min for lysozyme, and 20.4 and 21.7 min for lactoferrin, respectively. The assays may be performed in 30 min.
主要由白蛋白、溶菌酶和乳铁蛋白组成的人泪液蛋白，使用新型陶瓷羟基磷灰石柱通过高效液相色谱法进行测定。在室温下，使用从95:5% A/B缓冲液到0:100% A/B缓冲液（缓冲液A为蒸馏水；缓冲液B为含240 mM氢氧化钠的400 mM磷酸二氢钾）的20分钟线性梯度洗脱蛋白质。白蛋白在1.2分钟洗脱，溶菌酶在8.5分钟洗脱，乳铁蛋白分别在20.4分钟和21.7分钟洗脱。测定可在30分钟内完成。
