在用于ATP依赖性肌动蛋白-肌球蛋白滑动的力-运动测定系统中，对聚苯乙烯珠粒的肌球蛋白包被表面进行扫描电子显微镜观察。

Scanning electron microscopy of the myosin-coated surface of polystyrene beads in a force-movement assay system for ATP-dependent actin-myosin sliding.

作者信息

Takahashi I, Oiwa K, Kawakami T, Tanaka H, Sugi H

机构信息

Central Laboratory for Electron Microscopy, School of Medicine, Teikyo University, Tokyo, Japan.

出版信息

J Electron Microsc (Tokyo). 1993 Oct;42(5):334-7.

PMID:8106853

Abstract

We examined the myosin-coated surface of polystyrene beads, which slide on actin filaments in the presence of ATP with characteristics essentially similar to those of contracting muscle, using a high-resolution scanning electron microscope (JSM-890, JEOL). The bead surface was mostly covered with myosin filaments (diameter, 15-45 nm) with helically arranged myosin heads. Considering the amount of force generated by the myosin heads on the bead (approximately 10 pN), this result indicates that the bead movement may be caused by about 10 myosin heads within a short myosin filament segment, but not by randomly oriented myosin heads.

摘要

我们使用高分辨率扫描电子显微镜（JSM - 890，日本电子株式会社）检查了聚苯乙烯珠粒上包被肌球蛋白的表面，该表面在ATP存在的情况下能在肌动蛋白丝上滑动，其特性与收缩肌肉的特性基本相似。珠粒表面大部分被肌球蛋白丝（直径15 - 45纳米）覆盖，这些肌球蛋白丝带有呈螺旋排列的肌球蛋白头部。考虑到肌球蛋白头部在珠粒上产生的力的大小（约10皮牛），这一结果表明，珠粒的移动可能是由短肌球蛋白丝段内约10个肌球蛋白头部引起的，而不是由随机取向的肌球蛋白头部引起的。