Beck F, Tucci J, Senior P V
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.
J Reprod Fertil. 1993 Nov;99(2):343-52. doi: 10.1530/jrf.0.0990343.
Hybridization histochemistry and solution hybridization studies were performed to localize the expression of parathyroid hormone-related protein (PTHrP) during implantation in rats. Parallel studies were performed on rat uteri bearing oil-induced deciduomata and on cultured blastocysts. PTHrP mRNA begins to be expressed at day 5.5 of gestation by the uterine epithelium in the anti-mesometrial crypts marking the sites of implantation and in comparable regions during induction of deciduoma. Trophoblastic giant cells express the gene as soon as they are phenotypically recognizable both in vivo and in culture, but PTHrP mRNA cannot be detected in the early blastocyst or in cells of the inner cell mass. Decidual cells produce PTHrP mRNA both in normal gestation and after the induction of deciduomata. In each case, the expression of the gene in decidual cells follows its expression in uterine epithelium by 48 h. The uterine topographical location and time of expression of the PTHrP gene suggests that it plays a part in the implantation of the blastocyst.
进行了杂交组织化学和溶液杂交研究，以定位大鼠着床期间甲状旁腺激素相关蛋白（PTHrP）的表达。对携带油诱导蜕膜瘤的大鼠子宫和培养的囊胚进行了平行研究。PTHrP mRNA在妊娠第5.5天开始由子宫上皮在标记着床部位的反系膜隐窝以及蜕膜瘤诱导期间的相应区域表达。滋养层巨细胞一旦在体内和培养中表型可识别就表达该基因，但在早期囊胚或内细胞团细胞中未检测到PTHrP mRNA。蜕膜细胞在正常妊娠和蜕膜瘤诱导后均产生PTHrP mRNA。在每种情况下，蜕膜细胞中该基因的表达比子宫上皮中的表达晚48小时。PTHrP基因的子宫拓扑位置和表达时间表明它在囊胚着床中起作用。
