Raychoudhury S S, Thompson E W, Blackshaw A W, Irving M G
Division of Science and Technology, Griffith University, Brisbane, Queensland, Australia.
J Reprod Fertil. 1993 Nov;99(2):513-8. doi: 10.1530/jrf.0.0990513.
To determine whether Sertoli cells influence DNA synthesis by rat peritubular myoid cells in vitro, the effects of Sertoli cells on [3H]thymidine incorporation by peritubular myoid cells in a coculture situation were examined. Incubation of testicular peritubular myoid cells with Sertoli cells in coculture induced a significant increase in [3H]thymidine incorporation by peritubular myoid cells. This indicates a cell-cell cooperation between Sertoli and peritubular myoid cells in the testis in terms of DNA synthesis. Secreted factors from Sertoli cells, as tested in a parabiotic culture situation, also increased [3H]thymidine incorporation by peritubular myoid cells. Moreover, in terms of total cellular protein, cocultures of Sertoli cells and peritubular myoid cells resulted in a significant increase when compared with the monocultures, and this coculture effect substituted for the stimulatory response of serum on peritubular myoid cell monoculture. This study investigated the cooperative role of Sertoli cells and peritubular myoid cells in paracrine regulation of testicular functions.
为了确定支持细胞是否在体外影响大鼠睾丸肌样细胞的DNA合成，研究了在共培养条件下支持细胞对睾丸肌样细胞掺入[3H]胸腺嘧啶核苷的影响。睾丸肌样细胞与支持细胞共培养后，其掺入[3H]胸腺嘧啶核苷的量显著增加。这表明在睾丸中，支持细胞与睾丸肌样细胞在DNA合成方面存在细胞间合作。在联体共生培养条件下进行测试时，支持细胞分泌的因子也增加了睾丸肌样细胞对[3H]胸腺嘧啶核苷的掺入。此外，就总细胞蛋白而言，支持细胞与睾丸肌样细胞的共培养物与单培养物相比显著增加，并且这种共培养效应替代了血清对睾丸肌样细胞单培养的刺激反应。本研究探讨了支持细胞和睾丸肌样细胞在旁分泌调节睾丸功能中的协同作用。
