Jones C J, Aplin J D, Mulholland J, Glasser S R
Department of Pathological Sciences, University of Manchester, UK.
J Reprod Fertil. 1993 Nov;99(2):635-45. doi: 10.1530/jrf.0.0990635.
Lectin histochemistry was used to demonstrate changes in the surface glycan distribution of uterine stromal cells as they differentiate to form decidual cells. Decidualization was induced in hormone-treated, ovariectomized rat uteri by needle scratch. Uterine tissue from days 2 to 8 of deciduoma development was examined with a panel of lectins specific for terminal non-reducing structures in N- and O-linked classes of glycoprotein glycan, including alpha 2,3- and alpha 2,6-linked sialic acid residues. Immunostaining for desmin was used to identify decidual cells. An increase in N-linked glycans associated with the cell surface and recognized by lectins from Phaseolus vulgaris (leukoagglutinin) (l-PHA), Pisum sativum (PSA) and Triticum vulgaris (WGA) was found during the early growth of decidual cells. As decidualization progressed regionally from the antimesometrial to mesometrial uterus, an increase in alpha 2,3-linked sialic acid residues was followed by a loss of the alpha 2,6-linked form. The results suggest that as stromal cells differentiate, glycoprotein biosynthesis and glycosyl transferase activity are altered. These changes in patterns of glycosylation may give rise to altered decidual cell-matrix and cell-cell interactions during differentiation and play a role in the modulation of decidual cell interactions with trophoblast during early placentation.
凝集素组织化学被用于证明子宫基质细胞分化形成蜕膜细胞时其表面聚糖分布的变化。通过针刺划痕在激素处理的去卵巢大鼠子宫中诱导蜕膜化。使用一组针对N-和O-连接类糖蛋白聚糖末端非还原结构的凝集素，包括α2,3-和α2,6-连接的唾液酸残基，检查蜕膜瘤发育第2至8天的子宫组织。用结蛋白免疫染色来鉴定蜕膜细胞。在蜕膜细胞早期生长过程中，发现与细胞表面相关且被菜豆（白细胞凝集素）（l-PHA）、豌豆（PSA）和普通小麦（WGA）凝集素识别的N-连接聚糖增加。随着蜕膜化从子宫反系膜区域向系膜区域进展，α2,3-连接的唾液酸残基增加，随后α2,6-连接形式减少。结果表明，随着基质细胞分化，糖蛋白生物合成和糖基转移酶活性发生改变。这些糖基化模式的变化可能导致分化过程中蜕膜细胞-基质和细胞-细胞相互作用改变，并在早期胎盘形成过程中调节蜕膜细胞与滋养层细胞的相互作用中发挥作用。
