来自短杆菌的原儿茶酸3,4-双加氧酶的初步晶体学研究。

Preliminary crystallographic study of protocatechuate 3,4-dioxygenase from Brevibacterium fuscum.

作者信息

Earhart C A, Radhakrishnan R, Orville A M, Lipscomb J D, Ohlendorf D H

机构信息

Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455.

出版信息

J Mol Biol. 1994 Feb 11;236(1):374-6. doi: 10.1006/jmbi.1994.1143.

DOI:10.1006/jmbi.1994.1143

PMID:8107119

Abstract

The enzyme protocatechuate 3,4-dioxygenase from the Gram positive organism Brevibacterium fuscum crystallizes in the triclinic space group P1 with unit cell dimensions a = 96.1 A, b = 97.2 A, c = 118.1 A and alpha = 113.9 degrees, beta = 90.7 degrees, gamma = 117.8 degrees. The rod-like crystals diffract to 2.4 A resolution. Rotation function analysis suggests that there are six promoters arranged with local 32 symmetry in the asymmetric unit rather than the previously proposed pentameric complex.

摘要

来自革兰氏阳性菌短褐短杆菌的原儿茶酸3,4-双加氧酶在三斜晶系空间群P1中结晶，晶胞参数为a = 96.1 Å，b = 97.2 Å，c = 118.1 Å，α = 113.9°，β = 90.7°，γ = 117.8°。棒状晶体的衍射分辨率为2.4 Å。旋转函数分析表明，在不对称单元中有六个启动子以局部32对称排列，而不是先前提出的五聚体复合物。