通过电子显微镜观察枯草芽孢杆菌DNA在H2O和D2O中的热变性。
Thermal denaturation of B. subtilis DNA in H2O and D2O observed by electron microscopy.
作者信息
Izzo V, Fornili S L, Cordone L
出版信息
Nucleic Acids Res. 1975 Oct;2(10):1805-10. doi: 10.1093/nar/2.10.1805.
Abstract
In the present work we studied the initial part of thermal denaturation curves of B. subtilis DNA in normal and heavy water, observing, by electron microscopy, the "opening" of DNA as a function of temperature. The results support the hypothesis that D(2)O plays a stabilizing role on strands separation during thermal denaturation of DNA.
摘要
在本研究中，我们研究了枯草芽孢杆菌DNA在普通水和重水中热变性曲线的起始部分，通过电子显微镜观察DNA的“解链”随温度的变化情况。结果支持了这样的假设：在DNA热变性过程中，重水对链的分离起到稳定作用。
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