Kantar A, Oggiano N, Giorgi P L, Fiorini R
Department of Pediatrics, University of Ancona, Italy.
Pediatr Res. 1993 Dec;34(6):725-8. doi: 10.1203/00006450-199312000-00006.
Plasma membrane fluidity and heterogeneity of polymorphonuclear leukocytes (PMN) were investigated in seven children with primary ciliary dyskinesia (PCD) and 17 healthy controls. Membrane fluidity and heterogeneity were studied by measuring the steady state fluorescence anisotropy and fluorescence decay of 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated into PMN plasma membrane. Our results show an increase in membrane fluidity at the surface level of PMN from patients with PCD. Distribution analysis of TMA-DPH lifetime values indicate an increase in membrane heterogeneity in subjects with PCD. The observed changes in the physicochemical properties of the membrane could lead to alterations in the function of PMN from children with PCD.
对7名原发性纤毛运动障碍（PCD）患儿和17名健康对照者的多形核白细胞（PMN）的质膜流动性和异质性进行了研究。通过测量掺入PMN质膜中的1-（4-三甲基铵苯基）-6-苯基-1,3,5-己三烯（TMA-DPH）的稳态荧光各向异性和荧光衰减来研究膜流动性和异质性。我们的结果显示，PCD患者的PMN表面水平的膜流动性增加。TMA-DPH寿命值的分布分析表明，PCD患者的膜异质性增加。观察到的膜物理化学性质的变化可能导致PCD患儿的PMN功能改变。
