Caimi G, Lo Presti R, Montana M, Ferrara F, Ventimiglia G, Meli F, Catania A, Canino B
Istituto di Clinica Medica e Malattie Cardiovascolari, Università di Palermo, Italy.
Horm Metab Res. 1998 Feb;30(2):72-6. doi: 10.1055/s-2007-978838.
We evaluated polymorphonuclear (PMN) filtration parameters, membrane fluidity and cytosolic Ca2+ content in 21 normal subjects and in 18 type II diabetics with macrovascular complications (MVC). Evaluations were carried out at baseline and after in vitro activation prolonged for 5 and 15 min. PMA (4-phorbol 12-myristate 13-acetate) and fMLP (N-formyl-methionyl-leucyl-phenylalanine) were used as stimulating agents. TMA-DPH (1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene) was used as fluorescent probe for the membrane fluidity tests and Fura 2-AM for the cytosolic Ca2+ content. A significant variation was evident in PMN filtration parameters at 5 and 15 min. No variation was present in PMN membrane fluidity and cytosolic Ca2+ content in normals. In type II diabetics with MVC, we found an increase solely in PMN cytosolic Ca2+ content after PMA activation and an early decrease in PMN membrane fluidity and a late increase in PMN cytosolic Ca2+ content after fMLP activation. After PMA activation alone (at 15 min), PMN filtration distinguishes normals from type II diabetics with MVC. The PMN filtration parameters behave similarly in the two groups, but PMN membrane fluidity and cytosolic Ca2+ content behave differently.
我们评估了21名正常受试者以及18名患有大血管并发症（MVC）的II型糖尿病患者的多形核白细胞（PMN）过滤参数、膜流动性和细胞溶质Ca2+含量。评估在基线时以及体外激活延长5分钟和15分钟后进行。使用佛波酯（4-佛波醇12-肉豆蔻酸酯13-乙酸酯）和N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）作为刺激剂。使用1-[4-(三甲氨基)苯基]-6-苯基-1,3,5-己三烯（TMA-DPH）作为膜流动性测试的荧光探针，使用Fura 2-AM测定细胞溶质Ca2+含量。在5分钟和15分钟时，PMN过滤参数有明显变化。正常受试者的PMN膜流动性和细胞溶质Ca2+含量没有变化。在患有MVC的II型糖尿病患者中，我们发现经佛波酯激活后仅PMN细胞溶质Ca2+含量增加，经fMLP激活后PMN膜流动性早期降低，细胞溶质Ca2+含量后期增加。仅在经佛波酯激活后（15分钟时），PMN过滤可区分正常受试者和患有MVC的II型糖尿病患者。两组的PMN过滤参数表现相似，但PMN膜流动性和细胞溶质Ca2+含量表现不同。
