一个受发育调控的类碱性亮氨酸拉链基因及其在胚胎视网膜和晶状体中的表达。

Retinal Degenerations Research Center, Wilmer Institute, Johns Hopkins University, School of Medicine, Baltimore, MD 21287-9257.

Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1351-5. doi: 10.1073/pnas.91.4.1351.

Transcriptional regulators play important roles in the control of key developmental events. We have identified sw3-3, a likely candidate for such a function, in tissues of the eye and other neural organs. It encodes a basic-leucine zipper-like protein, in which two leucine zipper motifs flank a basic domain. The latter contains helix-disturbing amino acids such as glycine and proline, at positions occupied by conserved asparagine and alanine residues (respectively) in "conventional" basic-leucine zipper proteins. sw3-3 is widely expressed at early embryonic stages in the lens, retina, and other neural tissues and is down-regulated thereafter with a spatial and temporal pattern that correlates with the cessation of mitotic activity and the onset of cell migration and differentiation.

转录调节因子在关键发育事件的控制中发挥着重要作用。我们在眼睛和其他神经器官的组织中鉴定出了sw3-3，它可能具有这样的功能。它编码一种类碱性亮氨酸拉链蛋白，其中两个亮氨酸拉链基序位于一个碱性结构域两侧。在“传统”碱性亮氨酸拉链蛋白中，该碱性结构域相应位置分别被保守的天冬酰胺和丙氨酸残基占据，而此处含有如甘氨酸和脯氨酸等扰乱螺旋的氨基酸。sw3-3在胚胎早期在晶状体、视网膜和其他神经组织中广泛表达，此后随着有丝分裂活动的停止以及细胞迁移和分化的开始，其表达在时空模式上被下调。