Ishida K, Mizuno A, Sano T, Shi K, Shima K
Department of Laboratory Medicine, School of Medicine, University of Tokushima, Japan.
Acta Endocrinol (Copenh). 1993 Dec;129(6):585-93. doi: 10.1530/acta.0.1290585.
Pancreatic A-cell function in the newly developed Otsuka Long Evans Tokushima Fatty (OLETF) strain of non-insulin-dependent diabetes mellitus (NIDDM) rats was examined in relation to the morphological changes in their islets and the plasma glucagon responses to insulin-induced hypoglycemia and an arginine test by chronological studies in seven male OLETF and seven male non-diabetic control Long Evans Tokushima Otsuka (LETO) rats each at 10, 16 and 24 weeks of age and eight male OLETF rats that were placed in a cage with a wheel for exercising from 5 to 24 weeks of age. The hormonal contents and morphological features of the pancreas of these rats were examined. After iv injection of insulin, the plasma glucagon level rose significantly from the basal level in OLETF rats at 10 weeks old, but little if at all in those of 16 and 24 weeks old. The pancreatic A cells of LETO rats of all age groups responded equally well to glucopenia. The areas under the response curves of plasma glucagon (sigma delta IRG) during the 90 min of insulin-induced hypoglycemia were 14496 +/- 7860 vs 9588 +/- 3930, 2257 +/- 3018 vs 9235 +/- 5447 (p < 0.05) and 826 +/- 985 vs 9707 +/- 2510 (p < 0.01) ng.min-1.l-1 in OLETF rats vs LETO rats of 10, 16 and 24 weeks old, respectively. The plasma glucagon responses during the arginine test were higher in OLETF rats than in LETO rats at 10 and 16 weeks but not at 24 weeks of age.(ABSTRACT TRUNCATED AT 250 WORDS)
通过对7只雄性大冢长-艾-托卡ushima肥胖（OLETF）非胰岛素依赖型糖尿病（NIDDM）大鼠和7只雄性非糖尿病对照长-艾-托卡ushima大冢（LETO）大鼠在10、16和24周龄时进行的时间顺序研究，以及对8只5至24周龄置于带轮子笼子中运动的雄性OLETF大鼠的研究，探讨了新培育的OLETF品系NIDDM大鼠胰腺A细胞功能与胰岛形态变化以及血浆胰高血糖素对胰岛素诱导低血糖和精氨酸试验反应的关系。检测了这些大鼠胰腺的激素含量和形态特征。静脉注射胰岛素后，10周龄的OLETF大鼠血浆胰高血糖素水平从基础水平显著升高，但16周龄和24周龄的大鼠升高很少或几乎没有升高。所有年龄组的LETO大鼠胰腺A细胞对低血糖的反应同样良好。胰岛素诱导低血糖90分钟期间血浆胰高血糖素反应曲线下面积（σΔIRG）在10周龄、16周龄和24周龄的OLETF大鼠中分别为14496±7860、2257±3018和826±985 ng·min⁻¹·L⁻¹，在LETO大鼠中分别为9588±3930、9235±5447和9707±2510 ng·min⁻¹·L⁻¹（p<0.05和p<0.01）。精氨酸试验期间，10周龄和16周龄时OLETF大鼠的血浆胰高血糖素反应高于LETO大鼠，但24周龄时并非如此。（摘要截断于250字）
