Puka M, Lazarewicz J W
Department of Neurochemistry, Polish Academy of Sciences, Warsaw.
Acta Neurobiol Exp (Wars). 1993;53(4):511-6.
The uptake of 36Cl- into the fraction of membrane vesicles from rat and rabbit cortex and rabbit hippocampus was measured to investigate the selectivity of agonists and antagonists of inhibitory amino acid receptors coupled to chloride channels in the forebrain gamma-Aminobutyric acid (GABA) produced a dose-dependent 36Cl- uptake into membrane vesicles, whereas glycine and taurine were ineffective. The chloride uptake induced by GABA was inhibited in a dose-dependent manner by bicuculline and picrotoxin, established antagonists of GABAA receptors. GABA elicited chloride influx was also inhibited, with similar potency, by strychnine, thus indicating a limited selectivity of this glycine receptor antagonist.
测量了大鼠和家兔皮层以及家兔海马体膜囊泡部分对³⁶Cl⁻的摄取，以研究与前脑氯离子通道偶联的抑制性氨基酸受体激动剂和拮抗剂的选择性。γ-氨基丁酸（GABA）使膜囊泡对³⁶Cl⁻的摄取呈剂量依赖性，而甘氨酸和牛磺酸则无此作用。GABAA受体的既定拮抗剂荷包牡丹碱和印防己毒素以剂量依赖性方式抑制了GABA诱导的氯离子摄取。士的宁也以相似效力抑制了GABA引发的氯离子内流，从而表明这种甘氨酸受体拮抗剂的选择性有限。
