Domergue N, Pugniere M, Previero A
INSERM U 58, Montpellier, France.
Anal Biochem. 1993 Nov 1;214(2):420-5. doi: 10.1006/abio.1993.1517.
Amino acids dissolved in aqueous methanol (or ethanol) and treated with optically active menthyl chloroformate were converted into N-menthyloxycarbonyl methyl (or ethyl) ester derivatives within a few minutes at room temperature. The obtained diastereomeric derivatives, with the exception of arginine and histidine, had suitable gas chromatographic properties allowing enantiomeric analysis of a large number of proteinogenic and synthetic amino acids.
溶解于甲醇（或乙醇）水溶液中的氨基酸，用旋光性氯甲酸薄荷酯处理后，在室温下几分钟内就会转化为N-薄荷氧基羰基甲基（或乙基）酯衍生物。除精氨酸和组氨酸外，所得到的非对映体衍生物具有合适的气相色谱性质，可用于对大量蛋白质原氨基酸和合成氨基酸进行对映体分析。
