一种用于研究膜联蛋白与生物膜相互作用的电子顺磁共振方法。

An EPR method for studying annexin-biomembrane interaction.

作者信息

Megli F M, Selvaggi M, De Lisi A, Quagliareillo E

机构信息

Dipartimento di Biochimica e Biologia Molecolare, Universita' di Bari, Italy.

出版信息

Anal Biochem. 1993 Nov 1;214(2):557-65. doi: 10.1006/abio.1993.1538.

DOI:10.1006/abio.1993.1538

PMID:8109749

Abstract

Annexin p34 spin label has been used as a probe for studying annexin-membrane Ca-mediated interaction. The EPR spectrum of the purified probe is able to monitor the binding of the protein to phospholipid vesicles and isolated mitochondrial membranes, with increasing calcium concentration, as revealed by the calculated rotational correlation time. A novel mathematical approach is proposed to provide a direct estimation of annexin percentage binding to membranes, based on the correlation time calculated from experimental EPR spectra.

摘要

膜联蛋白p34自旋标记物已被用作研究膜联蛋白与膜的钙介导相互作用的探针。纯化后的探针的电子顺磁共振（EPR）光谱能够监测该蛋白与磷脂囊泡和分离的线粒体膜的结合情况，随着钙浓度的增加，通过计算得到的旋转相关时间可以证明这一点。本文提出了一种新颖的数学方法，基于从实验EPR光谱计算得到的相关时间，直接估算膜联蛋白与膜结合的百分比。