Kumar H D, Sharma V, Bisaria G P
Z Allg Mikrobiol. 1975;15(5):333-7.
Two unicellular algae, viz., Anacystis nidulans and Chlorella vulgaris, growing in polluted effluents, were isolated in unialgal and bacteria free culture. They were mutagenically exposed to ultraviolet radiation and variant strains endowed with differing capacities for growth and nutrient-gleaning were successfully isolated as distinct clones on agar plates. One such clone each of the two species was tested further and found stable. While these variant strains grew more slowly than untreated controls, statistically significant differences with respect to phosphate and nitrate uptake were found between treated and control strains of the two species.
从受污染废水中分离出两种单细胞藻类,即集胞藻(Anacystis nidulans)和小球藻(Chlorella vulgaris),并将它们培养成单种且无菌的培养物。它们经过紫外线诱变处理,在琼脂平板上成功分离出具有不同生长和养分获取能力的变异菌株,作为不同的克隆。对这两个物种各一个这样的克隆进行了进一步测试,发现它们是稳定的。虽然这些变异菌株的生长速度比未处理的对照慢,但在两个物种的处理菌株和对照菌株之间,发现了在磷酸盐和硝酸盐吸收方面具有统计学意义的差异。