Joshi M S, Rajagopalan K V
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Arch Biochem Biophys. 1994 Feb 1;308(2):331-4. doi: 10.1006/abbi.1994.1047.
All known molybdoenzymes other than nitrogenase contain the metal in association with molybdopterin or one of its dinucleotide variants. All eukaryotic molybdoproteins have been found to contain only molybdopterin, whereas the majority of bacterial enzymes contain one or another of the dinucleotides of molybdopterin. In contrast, xanthine dehydrogenase from Pseudomonas aeruginosa contains molybdopterin rather than a dinucleotide. To examine whether P. aeruginosa contains any dinucleotide of molybdopterin, cells were subjected to an analytical procedure which converts molybdopterin variants to the highly fluorescent Form A derivatives. The results showed that P. aeruginosa cells do contain molybdopterin guanine dinucleotide. The same procedure showed that rat liver does not contain any of the dinucleotides of molybdopterin.
除固氮酶外，所有已知的钼酶都含有与钼蝶呤或其一种二核苷酸变体结合的金属。已发现所有真核钼蛋白仅含有钼蝶呤，而大多数细菌酶含有钼蝶呤的一种或另一种二核苷酸。相比之下，铜绿假单胞菌的黄嘌呤脱氢酶含有钼蝶呤而非二核苷酸。为了检测铜绿假单胞菌是否含有钼蝶呤的任何二核苷酸，对细胞进行了一种分析程序，该程序可将钼蝶呤变体转化为高荧光的A形式衍生物。结果表明，铜绿假单胞菌细胞确实含有钼蝶呤鸟嘌呤二核苷酸。相同的程序表明，大鼠肝脏不含有钼蝶呤的任何二核苷酸。
