单克隆非特异性抑制因子（MNSF）抑制骨髓源性肥大细胞（BMMC）分泌白细胞介素4（IL4）。
Monoclonal non-specific suppressor factor (MNSF) inhibits the IL4 secretion by bone marrow-derived mast cell (BMMC).
作者信息
Nakamura M, Xavier R M, Tanigawa Y
机构信息
Department of Biochemistry, Shimane Medical University, Izumo, Japan.
出版信息
FEBS Lett. 1994 Feb 21;339(3):239-42. doi: 10.1016/0014-5793(94)80423-0.
We investigated whether monoclonal non-specific suppressor factor (MNSF) has an effect on the IL4 secretion by BMMC sensitized with anti-DNP IgE monoclonal antibody. The sensitized BMMC showed a maximum increase of IL4 secretion at 10 ng/ml antigen activation. When MNSF (10 U) was added, it remarkably suppressed the IL4 secretion (82 +/- 3%). MNSF also suppressed the IL4 secretion by the mast cell line, CFTL-15, but not CFTL-12. Northern blot analysis showed that, when the same amounts of MNSF were added, IL4 mRNA expression was decreased, suggesting that the suppression results from the inhibition of a transcript.
我们研究了单克隆非特异性抑制因子（MNSF）是否对用抗二硝基苯基（DNP）IgE单克隆抗体致敏的骨髓来源肥大细胞（BMMC）分泌白细胞介素4（IL4）有影响。致敏的BMMC在10 ng/ml抗原激活时IL4分泌出现最大程度增加。当加入MNSF（10 U）时，它显著抑制了IL4分泌（82±3%）。MNSF也抑制肥大细胞系CFTL-15分泌IL4，但不抑制CFTL-12。Northern印迹分析表明，当加入相同量的MNSF时，IL4 mRNA表达降低，提示这种抑制是由转录抑制导致的。
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