来自白羽扇豆的两个泛素::79个氨基酸延伸蛋白编码融合基因的克隆与鉴定。

Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus.

作者信息

Jacinto A, Neves A M, Vassilevskaia T D, Ricardo C P, Rodrigues-Pousada C

机构信息

Laboratório de Genética Molecular, Instituto Gulbenkian de Ciência, Oeiras, Portugal.

出版信息

Gene. 1994 Feb 25;139(2):201-5. doi: 10.1016/0378-1119(94)90755-2.

DOI:10.1016/0378-1119(94)90755-2

PMID:8112604

Abstract

Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

摘要

从白羽扇豆核DNA文库中分离出两个不同的泛素::79氨基酸（aa）延伸蛋白编码融合基因，并进行了测序。这两个基因都有465个核苷酸的开放阅读框，编码一个单一的泛素（Ub）单体，该单体与一个79个氨基酸的延伸（Ext）蛋白框内融合。编码的Ub推导氨基酸序列与其他植物Ub的氨基酸序列相同。编码的Ext蛋白是假定的核糖体蛋白，高度碱性，彼此相差2个氨基酸，并且与其他植物的Ext蛋白具有高度相似性。