Pickles J O
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
Hear Res. 1993 Dec;71(1-2):225-9. doi: 10.1016/0378-5955(93)90038-3.
Actin mRNA was characterised in hair-cell enriched fractions of the chick basilar papilla, by means of the reverse-transcription polymerase chain reaction technique. Primers were directed against the 3' untranslated portions of the actin mRNAs. Evidence for beta-cytoplasmic and gamma-cytoplasmic actin mRNA was found; no evidence was found for alpha-skeletal, alpha-cardiac or type 5 cytoplasmic actin mRNAs. Since beta-actin is known to form bundles of filaments whereas gamma-actin does not, this suggests that the hair-cell stereocilia are composed of beta-actin.
通过逆转录聚合酶链反应技术，对鸡基底乳头富含毛细胞的部分中的肌动蛋白mRNA进行了表征。引物针对肌动蛋白mRNA的3'非翻译部分。发现了β-细胞质和γ-细胞质肌动蛋白mRNA的证据；未发现α-骨骼肌、α-心肌或5型细胞质肌动蛋白mRNA的证据。由于已知β-肌动蛋白会形成细丝束，而γ-肌动蛋白则不会，这表明毛细胞静纤毛由β-肌动蛋白组成。
