Boccara M, Aymeric J L, Camus C
Laboratoire de Pathologie Végétale, Institut National de la Recherche Agronomique, Paris, France.
J Bacteriol. 1994 Mar;176(5):1524-6. doi: 10.1128/jb.176.5.1524-1526.1994.
The role of endoglucanases (endoglucanases Z and Y) in Erwinia chrysanthemi pathogenicity on Saintpaulia ionantha was assessed by mutagenizing cloned cel genes (celZ and celY) and recombining them with the chromosomal alleles. Strains with an omega interposon in celZ, a deletion in celY, or a double cel mutant were as virulent as the wild-type strain. However, in the strain with a deletion in celY, a delay in the appearance of symptoms was observed, and then maceration progressed as in plants infected with the wild-type strain, suggesting that E. chrysanthemi endoglucanases play a minor role in soft rot disease development.
通过对克隆的纤维素酶基因（celZ和celY）进行诱变并将其与染色体等位基因重组，评估了内切葡聚糖酶（内切葡聚糖酶Z和Y）在菊欧文氏菌对非洲紫罗兰致病性中的作用。celZ中有ω插入子、celY有缺失或双cel突变体的菌株与野生型菌株一样具有毒性。然而，在celY有缺失的菌株中，观察到症状出现延迟，然后软腐进程与感染野生型菌株的植物一样，这表明菊欧文氏菌内切葡聚糖酶在软腐病发展中起次要作用。
