双异丙酚对柯萨奇B原衣壳抗原性的稳定作用。

Stabilization of the antigenicity of coxsackie B procapsids by disoxaril.

作者信息

Frisk G, Halloran M, Didarholm H

机构信息

Department of Medical Virology, University of Uppsala, Sweden.

出版信息

J Virol Methods. 1993 Dec 15;45(2):235-9. doi: 10.1016/0166-0934(93)90107-3.

DOI:10.1016/0166-0934(93)90107-3

PMID:8113348

Abstract

Disoxaril added to cell cultures infected with coxsackie B virus serotypes 3 or 4 increased the yield and stabilized the antigenicity of the procapsids. In the absence of disoxaril, the procapsids lost their broad antigenicity after a short period of time. Prevention of this loss by disoxaril was observed in radioimmunoassays of IgM in sera from patients infected with various enteroviruses. The procapsids are useful in serological diagnosis of enterovirus infections, and the addition of disoxaril facilitates this technique.

摘要

将双异丙氧乙酰胺添加到感染柯萨奇B病毒3型或4型的细胞培养物中，可提高前衣壳的产量并稳定其抗原性。在没有双异丙氧乙酰胺的情况下，前衣壳在短时间后会失去其广泛的抗原性。在对感染各种肠道病毒的患者血清中的IgM进行放射免疫测定时，观察到双异丙氧乙酰胺可防止这种抗原性丧失。前衣壳可用于肠道病毒感染的血清学诊断，添加双异丙氧乙酰胺有助于这项技术。