重组痘苗病毒对小鼠巨细胞病毒糖蛋白H的表达

Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus.

作者信息

Rapp M, Lucin P, Messerle M, Loh L C, Koszinowski U H

机构信息

Department of Virology, University of Ulm, Germany.

出版信息

J Gen Virol. 1994 Jan;75 ( Pt 1):183-8. doi: 10.1099/0022-1317-75-1-183.

DOI:10.1099/0022-1317-75-1-183

PMID:8113726

Abstract

The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5.0 kb, which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity of the MCMV gH with a 87K envelope glycoprotein described previously by Loh and Qualtiere. Immunization of mice with the Vac-gH recombinant gave rise to an anti-gH serum, which neutralized MCMV without complement in vitro.

摘要

测定了小鼠巨细胞病毒（MCMV）史密斯株编码糖蛋白H（gH）的基因序列，并与MCMV K181株的gH序列进行了比较。转录分析表明，gH由一个5.0 kb的大mRNA编码，该mRNA在感染后期合成。构建了表达MCMV gH开放阅读框的重组痘苗病毒（Vac-gH）。抗MCMV血清从Vac-gH感染的细胞中沉淀出一种87K的蛋白质。与单克隆抗体的反应性表明MCMV gH与Loh和Qualtiere先前描述的87K包膜糖蛋白相同。用Vac-gH重组体免疫小鼠产生了抗gH血清，该血清在体外无补体的情况下中和了MCMV。