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丝裂原活化蛋白激酶激酶1（MKK1）受苏氨酸磷酸化负调控。

Mitogen-activated protein kinase kinase 1 (MKK1) is negatively regulated by threonine phosphorylation.

作者信息

Rossomando A J, Dent P, Sturgill T W, Marshak D R

机构信息

W. M. Keck Structural Biology Laboratory, Beckman Neuroscience Center, Cold Spring Harbor Laboratory, New York 11724.

出版信息

Mol Cell Biol. 1994 Mar;14(3):1594-602. doi: 10.1128/mcb.14.3.1594-1602.1994.

DOI:10.1128/mcb.14.3.1594-1602.1994
PMID:8114697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC358518/
Abstract

Mitogen-activated protein kinase kinase 1 (MKK1), a dual-specificity tyrosine/threonine protein kinase, has been shown to be phosphorylated and activated by the raf oncogene product as part of the mitogen-activated protein kinase cascade. Here we report the phosphorylation and inactivation of MKK1 by phosphorylation on threonine 286 and threonine 292. MKK1 contains a consensus phosphorylation site for p34cdc2, a serine/threonine protein kinase that regulates the cell division cycle, at Thr-286 and a related site at Thr-292. p34cdc2 catalyzes the in vitro phosphorylation of MKK1 on both of these threonine residues and inactivates MKK1 enzymatic activity. Both sites are phosphorylated in vivo as well. The data presented in this report provide evidence that MKK1 is negatively regulated by threonine phosphorylation.

摘要

丝裂原活化蛋白激酶激酶1（MKK1）是一种双特异性酪氨酸/苏氨酸蛋白激酶，已被证明作为丝裂原活化蛋白激酶级联反应的一部分，可被raf癌基因产物磷酸化并激活。在此，我们报告MKK1在苏氨酸286和苏氨酸292位点发生磷酸化后失活。MKK1在苏氨酸286位点含有一个p34cdc2（一种调节细胞分裂周期的丝氨酸/苏氨酸蛋白激酶）的共有磷酸化位点，在苏氨酸292位点有一个相关位点。p34cdc2在体外催化MKK1这两个苏氨酸残基的磷酸化反应，并使MKK1的酶活性失活。这两个位点在体内也会被磷酸化。本报告中的数据提供了证据，表明MKK1受到苏氨酸磷酸化的负调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/948be5d00895/molcellb00003-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/2f09d9167ef5/molcellb00003-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/6e85eb71bf8d/molcellb00003-0078-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/9a16c7d554c7/molcellb00003-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/77c10f2d1986/molcellb00003-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/663489b67e91/molcellb00003-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/4b4292a3b634/molcellb00003-0081-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/948be5d00895/molcellb00003-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/2f09d9167ef5/molcellb00003-0078-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/6e85eb71bf8d/molcellb00003-0078-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/9a16c7d554c7/molcellb00003-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/77c10f2d1986/molcellb00003-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/663489b67e91/molcellb00003-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/4b4292a3b634/molcellb00003-0081-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad5/358518/948be5d00895/molcellb00003-0082-a.jpg

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